In vitro Anticestode Activity of Painted Nettle Leaves Extract to Hymenolepis microstoma: Observation Using SEM

Study in uitro on the anticestodal activity of Coleus blu111ei leaves extracts against Hy111e11olepis 111icrosfolll11 was conducted. Leaves of C. blwuci were collected and extracted ·with hexane, chloroform, ethanol and water. Anthelmintic activity was evaluated with an assay using H. 111icrosfolllll in a serial microplate dilution method by determination of mortality time. The parasites were treated with varying concentrations, 10, 5, 2.5, 1, and 0.5 % of the plant extract. Scanning electron microscope analysis was performed to determine effect of painted nettle extract on the tegument and other superficial body chiuo efficacy.